MOTS-c Stability Study: Post-Reconstitution Degradation Analysis
Overview
This study was designed to evaluate the degradation profile of MOTS-c peptide post-reconstitution under refrigeration. The goal was to understand the peptide’s stability over time by assessing changes in volume and purity across four time points: Baseline, 7 Days, 14 Days, and 30 Days. All samples were stored at an ideal refrigerated range of 35–38°F (1.6–3.3°C) and reconstituted upon receipt to determine their post-reconstitution degradation behavior with below cadence.
Lab Reports:
Study Design
| Test # | Compound | Lot # | Storage | Condition | Reconstitution | Goal |
|---|---|---|---|---|---|---|
| Test 1 | MOTS-c | PC-Z2-MOT10 | Refrigerator | Baseline Sample Test | Upon Receipt of Sample | MOTS-c Degradation Post-Reconstitution |
| Test 2 | MOTS-c (7 Days) | PC-Z2-MOT10 | Refrigerator | 35–38°F (1.6–3.3°C) | Upon Receipt of Sample | MOTS-c Degradation Post-Reconstitution |
| Test 3 | MOTS-c (14 Days) | PC-Z2-MOT10 | Refrigerator | 35–38°F (1.6–3.3°C) | Upon Receipt of Sample | MOTS-c Degradation Post-Reconstitution |
| Test 4 | MOTS-c (30 Days) | PC-Z2-MOT10 | Refrigerator | 35–38°F (1.6–3.3°C) | Upon Receipt of Sample | MOTS-c Degradation Post-Reconstitution |
Results Summary
| Time Point | Avg. Volume (µL/mg) | Avg. Purity (%) | % Volume Change from Baseline | % Purity Change from Baseline |
|---|---|---|---|---|
| Baseline | 11.45 | 99.279 | 0.00% | 0.00% |
| 7 Days | 11.41 | 99.316 | -0.35% | +0.04% |
| 14 Days | 10.96 | 99.125 | -4.24% | -0.15% |
| 30 Days | 10.63 | 97.940 | -7.14% | -1.35% |
Phase-by-Phase Analysis
Baseline
The freshly reconstituted sample served as the control benchmark with:
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Volume: 11.45 µL/mg
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Purity: 99.279%
This established the standard for comparison throughout the study.
7 Days Post-Reconstitution
After one week of refrigeration:
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Volume slightly decreased to 11.41 µL/mg (−0.35% change).
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Purity increased marginally to 99.316% (+0.04%).
This indicated minimal degradation, supporting short-term refrigerated storage.
14 Days Post-Reconstitution
Two weeks in refrigeration showed more noticeable change:
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Volume decreased by 4.24% from baseline.
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Purity declined slightly to 99.125%, a −0.15% change.
While still within acceptable limits for research use, signs of degradation began to surface.
30 Days Post-Reconstitution
By the 30-day mark:
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Volume reduced to 10.63 µL/mg (−7.14% from baseline).
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Purity dropped to 97.940% (−1.35%).
The data suggests a steady degradation trend past the two-week mark, indicating that extended storage begins to compromise integrity.
Visual Trends
The following charts illustrate the degradation pattern in both volume and purity over time:
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Volume Over Time: A gradual decline from 11.45 to 10.63 µL/mg
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Purity Over Time: Maintained >99% for the first 14 days, then dipped below 98% by Day 30
Conclusion
This study demonstrates that MOTS-c peptide remains relatively stable for up to 14 days post-reconstitution when stored at recommended refrigeration temperatures (35–38°F / 1.6–3.3°C). During this period, the recorded changes were as follows:
- By Day 7: Volume decreased by 0.35%, Purity increased by 0.04%
- By Day 14: Volume decreased by 4.24%, Purity decreased by 0.15%
- By Day 30: Volume decreased by 7.14%, Purity decreased by 1.35%
These results provide a clear data set for researchers or formulators evaluating the reconstituted stability of MOTS-c under refrigeration. Based on this data, users may determine their own thresholds for acceptable purity and volume changes based on their specific applications.